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Eco-Responsible Protection: How Mushroom Chitosan is Revolutionizing Institutional Disinfection Practices

by Steve in Chitosan Applications May 31, 2024

What are the benefits of chitosan in disinfection applications?

1. Antimicrobial Properties: Chitosan has strong antimicrobial properties against a wide range of pathogens including bacteria, fungi, and viruses. This makes it an effective agent for disinfection purposes. 1

2. Biodegradability: As a natural biopolymer, chitosan is biodegradable. This quality makes it environmentally friendly compared to synthetic disinfectants, which can accumulate and cause environmental damage.

3. Non-toxicity: Unlike many chemical disinfectants, chitosan is non-toxic to humans and animals at the concentrations and forms used for disinfection. This makes it safe for use in various settings, including food preservation, water treatment, and surface sanitization.

4. Film-Forming Ability: Chitosan can form bioactive films and coatings that can be applied to surfaces for prolonged antimicrobial protection. This is particularly useful for keeping surfaces sterile over time without continuous application of disinfectant.

5. Metal Ion Chelation: Chitosan has the ability to chelate metal ions, which can enhance its antimicrobial effectiveness. The presence of chitosan can disrupt microbial cell walls by binding to essential metals required by bacteria and fungi.

6. Enhanced Efficacy with pH Sensitivity: Chitosan’s antimicrobial activity can be enhanced in acidic environments, which is beneficial since many disinfection scenarios involve controlled pH conditions.

These properties make chitosan an attractive option for disinfection in various applications, particularly where environmental impact and human safety are major factors.

What are the disinfection applications and products that use chitosan?

Chitosan, with its natural antimicrobial and biodegradable properties, is versatile and can be integrated into several of the disinfection application areas and product types. The addition ratio of chitosan and formulation guidelines can also vary widely based on the specific application, desired properties, and regulatory requirements. We have found, through lab tests at the University of Arizona, that mushroom chitosan, combined with citric acid, is lethal to pathogenic bacteria at a 1% concentration.

Here’s how it can be applied across these various sectors and some general guidelines and typical ratios used in different products and applications:

1. Healthcare Facilities, Schools, Jails, Government Buildings, Butcher and Meat packaging:
   • Surface Disinfectants: Chitosan can be used to formulate sprays and wipes that are effective against bacteria and viruses, suitable for cleaning patient rooms and other surfaces.
   • Instrument Disinfectants: It can be part of solutions for sterilizing medical instruments, offering a non-toxic and biodegradable alternative to harsh chemicals.
   • Addition Ratio: Around 1% to chitosan in the formulation plus a proton donator such as acetic acid or citric acid. For making quaternary chitosan antibacterial spray concentrate, no acid for proton donation is required.
   • Formulation Guideline: The product should be tested to ensure it does not leave residues that could interfere with healthcare operations. Compatibility with various surface materials is also essential.
   • Chitosan is the most powerful and effective non-toxic antimicrobial compound to come along since the discovery of quaternary ammonium compounds nearly a century ago.“Quats” were created to clean institutions and hospitals back in the day and still are. The problem is, they are toxic to people.It’s time to replace toxic antimicrobial “quat” products with non-toxic ones containing chitosan! To purchase quaternary chitosan in bulk at wholesale prices, click HERE

2. Water Treatment:
   • Chemical Disinfectants: Chitosan is useful in water purification systems for binding and neutralizing pathogens and heavy metals, enhancing the quality of drinking water without harsh chemicals.
   • Addition Ratio: Typically, 1 to 5 grams per liter of water, depending on the level of contamination and the purity required.
   • Formulation Guideline: Chitosan is often used as a flocculant; it helps to agglomerate suspended particles for easier removal. The pH of the water may need to be adjusted to optimize chitosan’s effectiveness, as it performs best in slightly acidic conditions.

3. Food and Beverage Industry:
   • Food Surface Sanitizers: Chitosan-based sanitizers can be used on food-contact surfaces to inhibit microbial growth, helping maintain hygiene in food processing areas.
   • Food Processing Disinfectants: As a natural antimicrobial, chitosan can be used to keep food processing environments sterile and free from contaminants.
   • Addition Ratio: About 0.5% to 2% chitosan in solution.
   • Formulation Guideline: Chitosan solutions for surface sanitizing should be compatible with food safety standards. Formulations might also include mild acids like acetic acid to enhance antimicrobial efficacy.

4. Public and Commercial Areas:
   • Air Disinfectants: Integrated into air filtration systems, chitosan can help in capturing and neutralizing airborne pathogens, suitable for places like schools and offices.
   • Addition Ratio: Not typically applied as a percentage; instead, chitosan is incorporated into the filter material during manufacturing.
   • Formulation Guideline: Ensure the chitosan is properly embedded within the filter media to maintain air flow and filtration efficiency.

5. Household Settings:
   • Multipurpose Disinfectants: Home cleaning products can include chitosan for its antimicrobial properties, making it ideal for kitchen, bathroom, and general household cleaning.
   • Laundry Sanitizers: Chitosan can be added to laundry detergents to help eliminate bacteria and viruses from fabrics during washing.
   • Addition Ratio: Usually around 0.5% to 2%.
   • Formulation Guideline: Products should be designed to maintain efficacy across a range of surface types and dirt levels. pH and stability tests are crucial to ensure long shelf life.

6. Industrial and Manufacturing:
   • Industrial Cleaners: Due to its effectiveness and safety, chitosan can be included in cleaners for use in manufacturing environments where non-toxic and eco-friendly solutions are preferred.
   • Addition Ratio: Coating solutions might contain 1% to 3% chitosan.
   • Formulation Guideline: In textiles, chitosan treatments should be applied so that they bond well with the fabric without affecting its breathability and texture.

7. Agriculture and Horticulture:
   • Greenhouse Sanitizers: Chitosan can be used to disinfect greenhouse surfaces and equipment, protecting plants from microbial infections.
   • Livestock Disinfectants: It can be used in formulations to sanitize animal housing, providing a safe environment for livestock.
   • Addition Ratio: Typically around 1% to 2% chitosan.
   • Formulation Guideline: Ensure that the chitosan does not negatively affect plant growth; compatibility with various plant species can vary.

General Considerations

• pH Sensitivity: Chitosan’s solubility and effectiveness are pH-dependent, with optimal performance in the pH range of 6.4 and below.
• Compatibility: Ensure that chitosan is compatible with other components in the formulation and does not react undesirably.
• Regulatory Compliance: Always consider local regulations regarding the use of biopolymers in consumer and industrial products, especially in the food and healthcare industries.

In each of these applications, chitosan offers a sustainable and effective alternative to traditional chemical disinfectants, aligning with growing demands for environmentally friendly and safe disinfection solutions. Meanwhile, these ratios and guidelines are starting points. Specific formulations often require thorough testing and optimization to meet the desired performance standards and regulatory requirements.

Which type of chitosan is suitable for the disinfection application & products?

Selecting the right type of chitosan for a specific application depends on the desired properties and the specifics of the application. Here’s a breakdown of which types might be best suited for the various applications and products:

1. Acid-Soluble Chitosan (Viscosity Range Specifics)

• 20-100 cps: Suitable for applications requiring quick and complete solubility, such as in water treatment and surface sanitizers where rapid action is essential.
• 100-500 cps: This medium viscosity is good for film-forming applications, such as coatings for food preservation or air filters, where a thicker layer might be beneficial for prolonged action.
• 500-1000 cps: Higher viscosity chitosan can be useful in applications where a more substantial gel or layer is beneficial, such as in wound dressings or agricultural sprays that need to adhere to surfaces longer.

2. Chitosan Hydrochloride

• This type is highly soluble in water and useful in applications where low pH environments are not practical. It’s ideal for healthcare applications, including instrument sterilization and surface disinfectants, where compatibility with a range of materials and conditions is necessary.

3. Chitosan Oligosaccharide

• Due to its smaller molecular size and high solubility, chitosan oligosaccharide is particularly effective in applications requiring rapid bioactivity. It is well-suited for water treatment, cosmetics, and potentially as an additive in air filtration systems to quickly neutralize pathogens.

4. Carboxymethyl Chitosan

• This derivative has enhanced solubility across a broader pH range, making it versatile for use in cosmetics and personal care products where a neutral pH is common. It’s also suitable for food surface sanitizers and household cleaners, offering effective microbial control without altering the sensory properties of food or damaging surfaces.

Application Recommendations:

• Water Treatment: Acid-soluble chitosan (20-100 cps) or chitosan hydrochloride for its solubility.
• Food and Beverage Industry: Carboxymethyl chitosan for surface sanitizers, and acid-soluble chitosan (100-500 cps) for coatings.
• Healthcare Facilities: Chitosan hydrochloride for its excellent compatibility and effectiveness.
• Air Filtration: Chitosan oligosaccharide for rapid action and high efficiency.
• Household and Industrial Cleaners: Carboxymethyl chitosan for its versatility and effectiveness in a range of pH environments.
• Textile Treatments: Medium to high viscosity acid-soluble chitosan (100-1000 cps) for durable coatings.
• Agricultural Applications: High viscosity acid-soluble chitosan (500-1000 cps) for prolonged adherence and effectiveness on plants and surfaces. Chitosan oligosaccharide sprays offer a sustainable and effective option for enhancing crop resilience and yield, making them a valuable tool in the arsenal of modern sustainable agriculture.

Each of these types of chitosan has distinct properties that make them suitable for specific applications, and choosing the right one depends on the detailed requirements of the application including environmental conditions, required longevity of action, and regulatory compliance.

What are the forms of chitosan used in disinfection applications?

In disinfection applications, chitosan can be used in various forms to maximize its antimicrobial properties. And each form of chitosan has specific applications where it can be most effective due to its unique properties. Here are some of the most common forms and a breakdown of suitable application areas for each form:

1. Chitosan Solutions: These are aqueous solutions where chitosan is dissolved, typically with the aid of acidic agents like acetic acid to ensure solubility. These solutions can be used as sprays or soaks for surface disinfection, including food contact surfaces and medical equipment.
   • Surface Disinfectants: Ideal for cleaning and disinfecting surfaces in healthcare settings, schools, and food preparation areas.
   • Water Treatment: Used in municipal water systems and pools to remove impurities and microbial contaminants.
   • Hand Sanitizers: Often formulated into liquid hand sanitizers for personal hygiene.

2. Chitosan Powders: Chitosan in powder form can be applied directly or used to formulate various products such as tablets, powders, or incorporated into other materials. It’s used for water treatment, agricultural sprays, and sometimes in air filters.
   • Agricultural Sprays: As a powder, chitosan can be mixed with water and sprayed onto plants to help with disease resistance and growth enhancement.
   • Industrial Disinfectants: Powder form can be used to create bulk disinfectant products for large scale industrial cleaning.

3. Chitosan Films and Coatings: Chitosan can be used to create thin films or coatings that are applied to surfaces or products. These films are particularly useful in food preservation, where they act as a barrier to microbial contamination and extend the shelf life of perishable goods.
   • Food Preservation: Applied as a coating on perishable foods to extend shelf life and prevent microbial growth.
   • Medical Coatings: Used on medical devices or implants to prevent bacterial infection and improve biocompatibility.

4. Chitosan Gels: Gels made from chitosan are used for wound dressing and skin sanitizing applications because of their ability to form protective barriers and promote healing while providing antimicrobial protection.
   • Wound Care: Gels are particularly useful for dressing wounds due to their protective, healing, and antimicrobial properties.

5. Chitosan Capsules or Soft Gels: These are used in water treatment applications, where they can be added to water systems to gradually release chitosan, helping to purify the water by binding with impurities and microbial contaminants.
   • Water Purification Tablets: Used in portable water purification systems for travelers or in emergency situations.
   • Controlled Release Applications: In agricultural settings, slow-release tablets can provide sustained delivery of chitosan to help with plant protection.

6. Composite Materials: Chitosan is often combined with other materials to enhance its properties. For example, it can be combined with other natural polymers, nanoparticles, or fibrous materials to be used in filters for air and water purification systems.
   • Air Filtration Systems: Composites can be used in HVAC systems to enhance air filtration capabilities.
   • Advanced Water Treatment: Used in specialized filters for removing more complex or smaller contaminants from water.

Each form of chitosan has specific application techniques and advantages, depending on the desired disinfection outcome and the properties of the area or product being treated. Each of these forms of chitosan leverages its natural antimicrobial and biocompatible properties, making them suitable for a wide range of applications across different sectors.

Chitosan, derived from sources like mushrooms, serves as an effective and eco-friendly disinfectant in various applications, leveraging its inherent antimicrobial properties. Its biodegradable nature makes it particularly appealing in environments where reducing chemical residues is crucial, such as in food processing and healthcare settings. In the food industry, chitosan is used as a coating for fruits and vegetables, not only to inhibit the growth of pathogens but also to extend shelf life by forming a semi-permeable barrier that controls moisture and gas exchange. Similarly, in healthcare facilities, chitosan solutions can be employed to sanitize surfaces, instruments, and even incorporated into hand sanitizers, providing a safe alternative to traditional chemical disinfectants, reducing the risk of allergic reactions and promoting a safer environment for both patients and staff.

The versatility of chitosan allows it to be adapted into various forms such as powders, gels, and films, each suitable for specific applications. For instance, chitosan films are used extensively in wound care products due to their ability to promote healing and prevent infections. These films form a protective barrier over wounds, optimizing moisture levels, and enhancing the natural healing process while preventing bacterial infections. In water treatment, chitosan’s effectiveness at binding with pollutants and pathogens is utilized to purify drinking water, making it safer for consumption without the use of harsh chemicals. Its ability to chelate metals and remove suspended solids from water further underscores its utility in environmental conservation efforts, highlighting chitosan’s role as a sustainable solution in the ongoing challenge of effective yet environmentally conscious disinfection practices.

References:

1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8303267/ 

Factors Influencing the Antibacterial Activity of Chitosan and Chitosan Modified by Functionalization

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